The Single-Column Adapter is compatible with LS, LD, or Whole Blood Columns. LS Columns are optimized for positive selection and depletion of strongly magnetic labeled cells. LD Columns are optimized for depletion and Whole Blood Columns for cell isolation directly from whole blood, bone marrow, and buffy coat.
Thus far, three major techniques have been used for cell separation: centrifugation-based methods, fluorescence-activated cell sorting (FACS), and magnetic cell sorting. FACS and magnetic cell sorting offer the advantage of a more specific isolation, as they use antibodies to select the target population.
When a column is placed in a MACS Separator, the column spheres amplify the magnetic field of the Separator by 10,000-fold, thus inducing a strong magnetic force within the column. Thereby, even cells that are only slightly labeled with MACS MicroBeads are retained in the column.
Column-based magnetic cell separation techniques involve passing a sample previously labeled with magnetic particles through a column matrix within a magnetic field. The column is filled with ferromagnetic spheres that become magnetized in the applied magnetic field, creating a localized magnetic field that can immobilize the magnetic particles .
Magnetic separators in column-based and column-free systems generate a comparable magnetic force. What makes the difference, is the column. When the column is placed in a MACS Separator, the magnetic field gets amplified by 10,000-fold, due to the ferromagnetic spheres that are packed in the column.
LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells.LS Columns can be used with the following separators:MidiMACS™ Separator QuadroMACS™ Separator SuperMACS™ II Separator, in combination with an LS Column AdapterMultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter | USA
MagCellect Cell Selection Kits provide a powerful tool for enriching cell populations by positive or negative selection without the need for specialized columns. Features. Beads have high binding capacity; Small beads in ferrofluid have no magnetic memory and do not induce cell damage; Cells can be separated to a very high purity in minutes